How do you actually use ERCC spike-ins for RNA-seq? (ALR Transformation?)

2022.01.17 15:49 o-rka How do you actually use ERCC spike-ins for RNA-seq? (ALR Transformation?)

I finally got my hands on a dataset with properly designed ERCC92 spike ins. The question is, how should I use these with ALR in theory?

The additive log-ratio transformation (alr), which allows the user to scale their data by a feature with an a priori known fixed abundance, such as a house-keeping gene or an experimentally fixed variable (e.g., a ThermoFisher ERCC synthetic RNA “spike-in”15), may provide a superior alternative. In contrast to clr, proportionality calculated with alr does not change with missing feature data because it effectively back-calculates the absolute feature abundance.
https://www.nature.com/articles/s41598-017-16520-0
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2022.01.17 15:49 peleg462 Dart monkey server goes hard tho

Dart monkey server goes hard tho submitted by peleg462 to battles2 [link] [comments]


2022.01.17 15:49 Bonus1Fact NEW: Waves from the Tonga tsunami are still being felt in Australia – and even a 50cm surge could knock you off your feet

NEW: Waves from the Tonga tsunami are still being felt in Australia – and even a 50cm surge could knock you off your feet submitted by Bonus1Fact to SaltyArmy [link] [comments]


2022.01.17 15:49 NinjaA55a55in89 Best Fire Dps

This doesn’t have to be specifically PvE or PvP just kinda overall. Polls only give so many options. Other characters in comments.
View Poll
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2022.01.17 15:49 liveinlivingcolor I'm hurting because of him, but I think he's stringing me along, but I also don't want to cut him off or just never reply him again

Last year I started talking to this guy. Pandemic was still bad so we never made plans to actually meet, but we kept talking, almost every day. (He invitede me for a trip, actually, but I had just started talking to him and wasn't feeling too confident about covid)
In December I invited him last minute for an event and then we actually met in person, he has funny, charming and didn't seem repulsed by me or anything, then we went out to eat and we shared an Uber.
I had a crush on him by then but meeting him was too much. I'm now crazy for the guy. I try to keep chatting other guys but they're not him. I had tickets to a movie three days after we first met and invited him, but he declined.
A few weeks after I made a joke of taking him out for ice cream but he either didn't understand or just chose to ignore it. So we still didn't see each other again.
Sometimes he goes missing and stops replying. Most times I keep quiet because people get busy or just don't feel like talking and just wait a few days or a week or so and he gets back. But he went missing at the end of last year and I thought he would send me a happy christmas or a happy new year and just never did and I don't know why I was so bummed about it.
So I did a combo of bad decisions: I went out (and made out) with another guy thinking it would be fine but talking to this other guy I just missed the first one more. And then I waited a few days more, and basically wrote a text saying that I had a crush on him and that I knew I had no right to ask anything of him but that he could tell me if he didn't want talk to me anymore and I'd understand because it feels awful waiting or if he wanted to try this in any way.
Basically he replied and said he never got a notification (but he's on instagram all the time, he couldn't have missed it), but he said he was sorry, that I wasn't being unfair, that we got close, that he knows I have some kind of feelings for him (and I said he didn't need to say sorry). He said he didn't "feel" any special feelings for me when we met.
He also said that he has some sort of crush on a guy at his work who he has nothing in common with (his words), that they flirted on a trip (but nothing happened and the guy took it back because he's afraid to come out) and that he likes me, but he thinks it's as a friend.
I read that and took a while to reply and I said I would love to have him as a friend, and then a few days after I basically took everything back, and said I confused things, that I was just lonely and thought I had feelings for him and that I could have them eventually but that I had just met him once.
And that seemed right at the moment, but I'm trying to at least put some distance and not be at my phone just waiting for his replies (we are talking about something else, that was a few days ago) and stories but I just miss him. I just want to reply to him immediately and then wait some hours or days for his reply.
I don't want to be an asshole to a nice guy and just never reply him, but I don't think I could tell him to not talk to me again. I would like to think he would care if I just blocked him on instagram, but part of me feels he wouldn't.
Am I being crazy? What would you do?
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2022.01.17 15:49 SnooPredictions5092 Hi, pm me ;)

Hi, pm me ;) submitted by SnooPredictions5092 to SugarDaddyMeet_Sites [link] [comments]


2022.01.17 15:49 olympia_rose source: trust me babes

source: trust me babes submitted by olympia_rose to SCP [link] [comments]


2022.01.17 15:49 Marquis-de-Camembert Bild "Zeitung" mal wieder kriegsgeil. Drecksblatt.

Bild submitted by Marquis-de-Camembert to haltdiefressebild [link] [comments]


2022.01.17 15:49 Jade_Crystalstaring Baby Drones: Baby J

Baby Drones: Baby J submitted by Jade_Crystalstaring to MurderDrones [link] [comments]


2022.01.17 15:49 crapple21 Can I join the cool kids club now that I have a lift and bigger tires?

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2022.01.17 15:49 susubeansu PoOT: Laggy running?

Does anyone else lag like crazy while running in a straight line? Just started playing two days ago and my character ran fine previously but I notice she lags now.
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2022.01.17 15:49 The-Beefy-Cow BIFI on Metis. Stake BIFI. Earn METIS.

BIFI on Metis. Stake BIFI. Earn METIS. submitted by The-Beefy-Cow to METIS_IO [link] [comments]


2022.01.17 15:49 WalkureARCH Nintendo warns of spoofed sites pushing fake Switch discounts

Nintendo warns of spoofed sites pushing fake Switch discounts submitted by WalkureARCH to cybersecurity [link] [comments]


2022.01.17 15:49 kiingtiger_ what lens do you think fuji is releasing next? and when?

I really want to push the button and buy the 27mm WR pancake, but kinda hesitant because what if fuji releases another flat/compact lens. Do you think this is a possibility?
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2022.01.17 15:49 Jason_Poon NFT GIVEAWAY🎁 LIKE AND DROP YOUR WALLET ADDRESS!

NFT GIVEAWAY🎁 LIKE AND DROP YOUR WALLET ADDRESS! submitted by Jason_Poon to NFTExchange [link] [comments]


2022.01.17 15:49 JimboFive H: Unrolled Enclave Flamer. W: Cap offers

Aligned Flamer Barrel, Stabilized Stock, Reflex Sight
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2022.01.17 15:49 ScottyBarr Phenibut Precise Dosing

Hey all! Love to everyone from Phoenix! I am tapering off phenibut. I have the powder with a 250 mg scooper. My question is: How are people precisely cutting the dose by 50 or 100 mg? How do you measure the powder? Thank you as always!
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2022.01.17 15:49 BenHey Worth the read for any true Anti work people.

Worth the read for any true Anti work people. submitted by BenHey to antiwork [link] [comments]


2022.01.17 15:49 RavenNight16 Made some little greeting card decorations in my class! I also turned one of the butterflies into a doll-sized necklace.

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2022.01.17 15:49 bot725 Yea......I am a little bit crazy, but I still love Silver.

Yea......I am a little bit crazy, but I still love Silver. submitted by bot725 to Wallstreetsilver [link] [comments]


2022.01.17 15:49 Estifanostiruneh Unbelievably events captured on security camera

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2022.01.17 15:49 Constant-Weird81 dm me to jerk to her together

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2022.01.17 15:49 Bonus1Fact NEW: Motion of stars holds clue of dark matter shape in barred galaxies

NEW: Motion of stars holds clue of dark matter shape in barred galaxies submitted by Bonus1Fact to SaltyArmy [link] [comments]


2022.01.17 15:49 ligmanubr Retroativo de dissidio

Boa tarde. Gostaria de saber de podem me ajudar com uma dúvida sobre retroativo de dissidio. Fui demitida dia 30/06/2021 e recentemente a empresa onde trabalhei fez o pagamento do dissidio retroativo que estrava atrasado, de vigência 09/2020 a 08/2021. Eu achei que teria direito ao pagamento integral da diferença do reajuste do salário de todos os meses que trabalhei neste período registrada (9 meses).
Porém o contador da empresa me disse que como fui demitida em Junho eu não tenho direito ao dissidio retroativo, apenas ao recalculo de minha rescisão. Essa informação é verdadeira?
Me mandaram este artigo como argumento:
Boa tarde.
Conforme dito em e-mail anterior o funcionário demitido não tem direito ao dissidio retroativo e sim ao recalculo da rescisão. O reaguste aplicado foi de vigência 09/2020 a 08/2021 e não referente ao ano de 2020. Segue abaixo base legal das informações acima:
De acordo com o artigo 10 da Lei n° 10.192/2001, os salários dos empregados serão reajustados na respectiva data base anual por meio de livre negociação coletiva.
No entanto, em alguns casos a negociação coletiva poderá demorar, dessa forma, o acordo ou a convenção coletiva são publicados posteriormente a data base da categoria, ensejando ao empregado o pagamento de diferenças salariais retroativas a competência da data base.
Logo, se o empregado for demitido sem justa causa, após a data base em que o acordo ou a convenção estejam atrasados, quando o instrumento coletivo for publicado caberá ao empregador fazer o pagamento de uma rescisão complementar ao empregado sobre as diferenças salariais calculadas em rescisão.
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2022.01.17 15:49 ventilatorisufora Raspudić: Zašto prosječan Hrvat ne trguje obveznicama? Jer to kod nas nije fer

Raspudić: Zašto prosječan Hrvat ne trguje obveznicama? Jer to kod nas nije fer submitted by ventilatorisufora to croatia [link] [comments]


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